An actinomycete strain of Streptomyces sp. Je 1-93: a producer of antifungal antibiotics

Screening new naturally occurring biologically active compounds is an effective strategy for creating a portfolio of platforms developing chemical agents against multidrug-resistant microbial strains. Actinomycetes are extremely prolific source structurally diverse secondary metabolites, most which have pharmaceutical or biotechnological significance. Among them, the genus Streptomyces stands out, producing about 55 % all known antibiotics. However, due to significant redisco­very already compounds, especially among actinomycetes, rate discovery antibiotics has slowed considerably. Today, there growing interest in screening from poorly studied and extreme habitats. In this study, we demonstrated phylogeny, bioactivity dereplication metabolites Je 1-93 strain isolated rhizosphere soil juniper (Juniperus excelsa Bieb.). Phylogenetic analysis based on nucleotide sequence 16S rRNA gene allowed its identification showed greatest similarity with S. hydrogenans CA04 (100 identity). Analysis antimicrobial activity strong antifungal reference Candida albicans ATCC 885-653 as well multi-resistant C. №12 strain, resistant nystatin, amphotericin B, clotrimazole, itraconazole, ketoconazole fluconazole. To identify that probably provide activity, analysed produced by sp. 1-93. facilitate dereplication, obtained extracts were separated size-exclusion chromatography column filled Sephadex LH-20. Methanol was used mobile phase. As result database natural (Dictionary Natural Products), antibiotic antimycins found extract they high probability providing strain.